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Quality Control of Tryptic Digestion



  • Digestif comprises a soluble recombinant protein scaffold + 11 artificial peptides (iRT) with good ionization
  • Amino acids flanking iRT cleavage site either favour or hinder protease cleavage.
  • Retention time & relative intensity pattern of released iRT can assess the quality of sample workup, the extent of digestion, LC-MS performance & inter/intra-lab variability
  • Reference: DIGESTIF: A Universal Quality Standard for the Control of Bottom-Up Proteomics Experiments. J Proteome Res. 2015 Feb 6;14(2):787-803