Targeted Protein Quantification

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Digestif 13C15N

Application

  Quality controls of tryptic digestion

Specific labelling

  Arginine: 13C6,15N4, >99%

Lysine: 13C6,15N2, >99%

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The DIGESTIF standard consists of a soluble recombinant protein scaffold to which a set of 11 artificial peptides (iRT peptides) with good ionization properties has been incorporated. In the protein scaffold, the amino acids flanking iRT peptide cleavage sites were selected to either favor or hinder protease cleavage.

After sample processing, the retention time and relative intensity pattern of the released iRT peptides can be used to assess the quality of sample workup, the extent of digestion and the performance of the LC-MS system. Thus, DIGESTIF can be used to standardize a broad spectrum of applications, ranging from simple replicate measurements up to large-scale biomarker screenings in biomedical applications.