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Analytical services
& LC-MS assay development

Promise Proteomics brings a wealth of experience in helping its customers to develop customised quantitative LC-MS solution.

Studies have included biomarker evaluation & validation, pharmacokinetic studies of biotherapeutics, stability studies of proteins and more. LC-MS method development is based on high-level expertise in protein sciences and analytics and utilises up-to-date LC-MS instruments dedicated to protein quantitation.


  • Development of sample preparation protocols
  • Full development of LC-MS assays
  • Validation according to EMA/FDA guidelines
  • Analysis of large samplesets
  • Unique development programs tailored to client goals
  • Custom feasibility programs with milestones adapted to budget
  • A dedicated team that remains in contact
  • Optimized lead times

Key aspects of our LC-MS assay development

  • Offers optimal specificity
  • Improves quantification data
  • Gives absolute accuracy
  • Antibody-free sample preparation Reduces associated timelines and cost
  • Multiplex capability for protein analysis
  • Fast development times
  • Compatible with most of the biological matrices e.g. serum, plasma, CSF, cell lysate
  • Methods can be transferred to CROs to move from R&D stage into pre-clinical and clinical phases

Quality control procedures
performed on all proteins

Standard analyses

  • AA sequence (LC-MS)
  • Isotopic incorporation (LC-SRM)
  • Concentration (UV + BCA)
  • Purity (SDS-PAGE and/or RP-HPLC)
  • Optional intact mass analysis (LC-MS)
  • Proteins are delivered after removal of the purification tag(s) With tags, any analysis performed by Bradford protein assay is less clear

Optional analyses

  • Concentration (Amino acid analysis)
  • AAA determination performed after verification of purity & intact sequence Contaminants or additional AA can dramatically effect concentration
  • Determination of disulfide bonds (LC-MS)
  • Structural characterization e.g. analysis of post-translational modifications