PROMISE has developed a unique solution, dedicated to therapeutic drug monitoring of monoclonal antibodies, combining a full-length labelled mAb standard, a generic preparation process for human serum preparation, and a targeted LC-MS approach. The complete solution developed by PROMISE allows to implement rapidly therapeutic monitoring of mAbs in your lab using LC-MS.
Reagents & Consumables provided:
- Digested Trastuzumab & Cetuximab light antibodies
- Consumables, reagents & solutions for sample preparation, including trypsin.
- Quality controls for sample preparation
Support provided:
- days training on site.
- Sample preparation protocol & LC-MS methods.
Solutions & Equipments required:
- Solvents for LC-MS analysis: formic acid, acetonitrile.
- Vacuum manifold + pump (suited for 96 well plates)
- Speed-vacuum or gas evaporator
- 37°C oven or thermomixer (for sample digestion).
- LC-MS instrument